Migi (Dog) CDV/CPV/CAV Ab Rapid Quantitative Test Kit

Catalog Number: MG-CA-R032

Product Specifications

Attribute	Value
Place of Origin	China
Classification	Specific Reagents
CAT No.	MG-CA-R032
Package	Box
Appearance	Liquid
Specification	10T
Storage	Room Temperature
Shelf life	2 years
MOQ	10 boxes
Suitable for	Veterinary
Grade	Reagent Grade

Specification

10 Tests/Box Cat. No.: MG-CA-R032

Intended Use

The CDV/CPV/CAV Ab test is a fluorescence immunoassay used in conjunction with an Immunofluorescence Analyzer for the quantitative measurement of antibodies against canine distemper virus (CDV), canine parvovirus (CPV), and canine adenovirus (CAV) in serum or plasma samples. This test can provide reference levels of antibody titers after vaccination to determine whether revaccination is necessary. It is also used as an aid in diagnosing and monitoring infections of CDV, CPV, and CAV. For in vitro diagnostic use only. For professional use only.

Clinical Significance

Canine distemper virus (CDV), canine parvovirus (CPV), and canine adenovirus (CAV) are the three core components of the standard canine vaccination protocol. Measuring antibody titres against all three simultaneously offers a convenient assessment of overall immune status.

A single quantitative triple test supports confirmation of vaccination efficacy, identification of gaps in protection, and optimisation of booster schedules for breeding and boarding facilities.

Test Principle

This test employs a quantitative double antigen sandwich fluorescence immunoassay. The fluorescent signal intensity corresponds to the amount of CDV-Ab, CPV-Ab, and CAV-Ab captured, with the concentration expressed as Tu/ml.

Materials

Materials Provided 10 individual sealed pouches, each containing: Test Device for CDV-Ab, CPV-Ab, and CAV-Ab Desiccant pouch One ID Chip Instructions for Use 10 tubes of CDV/CPV/CAV Ab Sample Buffer 10 Pipette Tips Materials Required but Not Provided Immunofluorescence Analyzer (for reading test results) Timer Pipette (already provided) Centrifuge (not typically required for antibody tests)

Storage and Stability

Store the test kit at 4°C to 30°C until the expiration date. Once opened, operate the test device at 18°C to 28°C. Perform the test within 30 minutes of opening the pouch.

Specimen Collection and Preparation

The test can be conducted using serum or plasma (EDTA anticoagulant is recommended). Separate serum or plasma from blood within 3 hours of collection. Avoid severely hemolyzed specimens. Perform the test immediately after specimen collection. If testing cannot be done within 3 hours, store the specimen at 2°C to 8°C for no more than 72 hours. For long-term storage, freeze at -20°C or lower. Bring all materials to room temperature before use. Thaw frozen specimens completely and mix well. Avoid repeated freeze-thaw cycles.

Test Procedure

Preparation: Set the Test Device on a clean, level surface. Insert the ID Chip into the analyzer and ensure the Test Device lot number matches the ID Chip. Sample Preparation: Pipette 20 μl of the prepared sample into the CDV/CPV/CAV Ab Sample Buffer and mix gently. Avoid vigorous agitation and foaming. Running the Test: Choose between the Standard Test or Quick Test :

• (a) Standard Test:
• (b) Quick Test: Pipette 100 μl of the mixed sample into the sample well (S) of the CPV-Ab Test Device. Click “Quick Test” and start the timer immediately. Leave the Test Device at room temperature (18°C–28°C) for 10 minutes. Insert the Test Device into the meter holder and click “Start Test.” Select the sample type “Serum/Plasma.” Repeat for the CDV-Ab and CAV-Ab Test Devices. Pipette 100 μl of the mixed sample into the sample well (S) of the CPV-Ab Test Device. Click “Standard Test” and insert the CPV-Ab Test Device into the meter holder. Select the sample type “Serum/Plasma.” The meter will automatically start and read the result. Repeat the same steps for the CDV-Ab and CAV-Ab Test Devices. Results: Results are displayed on the main screen. Results can be printed automatically or by clicking “Print.”

Quality Control

Each CDV/CPV/CAV Ab Rapid Quantitative Test includes an internal control for routine quality assurance. If the internal control fails, the meter will display an error message, indicating that the test should be repeated.

Interpretation of Results

Detection range: CPV-Ab: 4.5–640 Tu/ml CDV-Ab: 5.0–640 Tu/ml CAV-Ab: 4.0–640 Tu/ml Reference Range: CPV-Ab (Tu/ml) CDV-Ab (Tu/ml) CAV-Ab (Tu/ml) Level Suggestion ≤9 ≤10 ≤8 0 No antibody protection, vaccination recommended. 9–50 10–20 8–18 1 Weak antibody protection, consider booster vaccination. 50–150 20–50 18–40 2 Moderate protection, monitor antibody levels every 6 months. 150–300 50–100 40–80 3 Moderate antibody protection, and strengthen antibody level monitoring, once every 6 months or so. 300-500 100-200 80-160 4

500 200 160 5 Strongest protection, annual testing recommended. Note: If the pet has not been vaccinated but shows antibody levels above level 1, it may indicate past infection. Antibody levels may vary due to individual immune responses to vaccination.

Warnings and Limitations

For in vitro diagnostic use only. Check packaging and labels before use. Do not use if damaged. Do not use the test device after the expiration date. Use a new pipette tip for each specimen. Procedural errors or substances not listed may cause inaccurate results.