Migibio logo Migibio Request a Quote
Migi (Dog) Canine Toxoplasma Gondii Antibody(cTOXO-Ab)Rapid Quantitative Test Kit

Migi (Dog) Canine Toxoplasma Gondii Antibody(cTOXO-Ab)Rapid Quantitative Test Kit

Catalog No.: MG-CA-R026

Migi (Dog) Canine Toxoplasma Gondii Antibody(cTOXO-Ab)Rapid Quantitative Test Kit — a quantitative fluorescence immunochromatography (FICT) rapid test for canine diagnostics, delivering results in minutes with pg/ml-level sensitivity. CE/ISO9001-compliant manufacturer. Cat. No. MG-CA-R026.

Catalog Number: MG-CA-R026

Product Specifications

AttributeValue
Place of OriginChina
ClassificationSpecific Reagents
CAT No.MG-CA-R026
PackageBox
AppearanceLiquid
Specification10T
StorageRoom Temperature
Shelf life2 years
MOQ10 boxes
Suitable forVeterinary
GradeReagent Grade

Specification

10 Tests/Box Cat. No.:

Mg-Ca-R026

Intended Use

The cTOXO-Ab test is a fluorescence immunoassay designed for the quantitative determination of Toxoplasma gondii antibodies in canine serum or plasma. It aids in diagnosing and monitoring canine Toxoplasma gondii infections. For in vitro diagnostic use only. For professional use only.

Clinical Significance

Toxoplasma gondii is a zoonotic protozoan parasite that can cause clinical disease in dogs and significant public health concern. Serological detection of antibodies indicates exposure and helps assess infection status.

Quantitative cTOXO antibody measurement supports diagnosis of clinical toxoplasmosis and screening of at-risk animals, informing preventive advice for owners.

Test Principle

This test utilizes a quantitative double antigen sandwich fluorescence immunoassay. The fluorescent signal intensity reflects the amount of cTOXO-Ab captured, and the concentration is expressed in IU/ml.

Materials Provided

Contents Quantity Individual Sealed Pouches 10 – Test Device 1 per pouch – Desiccant Pouch 1 per pouch ID Chip 1 Instructions for Use 1 cTOXO-Ab Sample Buffer Tubes 10 Pipette Tips 10 Materials Required But Not Provided: Immunofluorescence Analyzer Timer Pipette Centrifuge

Storage and Stability

Store the test kit at 4–30°C until the expiration date. Perform the test at 18–28°C after opening the Test Device. Once opened, use the Test Device within 30 minutes.

Specimen Collection and Preparation

The test can be performed using serum or plasma (EDTA anticoagulant recommended). Separate serum or plasma from blood within 3 hours of collection. Severely hemolyzed specimens should be replaced with fresh ones. It is ideal to perform the test immediately after specimen collection. If the test cannot be performed within 3 hours, store the specimen at 2–8°C for up to 72 hours. For long-term storage, freeze below -20°C. Bring all materials to room temperature before use. Ensure frozen samples are completely thawed and mixed thoroughly. Avoid repeated freezing and thawing. Only clear, non-hemolyzed specimens should be used.

Test Procedure

Refer to the Immunofluorescence Analyzer Operation Manual for detailed instructions. Place the Test Device on a clean, flat surface. Insert the ID Chip into the analyzer and click “Read ID Chip.” Ensure the Test Device lot number matches the ID Chip. Pipette 50 μL of the prepared sample into the cTOXO-Ab Sample Buffer and mix gently, avoiding vigorous agitation and foaming. Add 100 μL of the mixed sample to the sample well (S) on the Test Device. Avoid bubbles. Test Modes: Standard Test : Click “Standard Test,” insert the Test Device into the meter after adding the sample, and click “Start Test.” Select “Serum/Plasma” as the sample type. The meter will automatically read and display the test results. Quick Test : Click “Quick Test” and start the timer immediately after adding the sample to the well. Let the Test Device sit for 10 minutes at room temperature (18–28°C). Insert the Test Device into the meter and click “Start Test.” Select “Serum/Plasma” and the device will display the result. Results can be displayed on-screen or printed.

Quality Control

Each cTOXO-Ab test contains an internal control for routine quality checks. If an invalid control result occurs, the meter will display an error message, and a new test should be performed.

Interpretation of Results

The detection range of cTOXO-Ab in canine serum or plasma is 2.5–320 IU/ml. Result (IU/ml) Interpretation ≤ 5 Negative (-) 5–10 Suspicious (±) 10–25 Weak Positive (+) 25–100 Medium Positive (++)

100 Strong Positive (+++) Each laboratory should establish a reference range representative of its population.

Warnings and Limitations

For in vitro diagnostic use only. Inspect packaging and labels before use. Do not use if damaged or expired. Use a fresh pipette tip for each specimen. Errors may occur due to procedural issues or interfering substances not listed in this document.