Migi (Dog) Canine Progesterone (cProgesterone) Rapid Quantitative Test Kit

Catalog Number: MG-CA-R020

Product Specifications

Attribute	Value
Place of Origin	China
Classification	Specific Reagents
CAT No.	MG-CA-R020
Package	Box
Appearance	Liquid
Specification	10T
Storage	Room Temperature
Shelf life	2 years
MOQ	10 boxes
Suitable for	Veterinary
Grade	Reagent Grade

Specification

Package Size : 10 Tests/Box Catalog Number : MG-CA-R020

Intended Use

The cProgesterone test is a fluorescence immunoassay used with an Immunofluorescence Analyzer for the quantitative determination of progesterone concentration in canine serum or plasma. Clinical Applications : Determining optimal breeding dates. Predicting parturition dates or the timing of a Cesarean section. Detecting reproductive disorders such as split heat, delayed puberty, silent estrus, or hypoluteidism. For in vitro diagnostic use only . For professional use only.

Clinical Significance

Canine progesterone measurement is the cornerstone of reproductive management in breeding bitches. Serial progesterone testing enables precise determination of the fertile window and optimal mating or insemination timing.

Quantitative cProgesterone measurement supports ovulation timing, prediction of whelping dates, and diagnosis of reproductive abnormalities such as luteal insufficiency.

Test Principle

Utilizes a quantitative competitive fluorescence immunoassay technique. The fluorescent signal intensity correlates with the amount of cProgesterone captured, with results expressed in ng/ml or nmol/L.

Materials Provided

Contents Quantity Individual Sealed Pouches 10

• • Test Device Per pouch
• • Desiccant Pouch Per pouch ID Chip 1 Instructions for Use 1 cProgesterone Sample Buffer Tubes 10 Pipette Tips 10 Materials Required but Not Provided: Material Quantity Immunofluorescence Analyzer 1 Timer 1 Pipette 1 Centrifuge 1

Storage and Stability

Store the test kit at 4–30℃ until the expiration date. Operate the test at 18–28℃ after opening the Test Device. Perform the test within 30 minutes of opening the pouch.

Specimen Collection and Preparation

Suitable for use with serum or plasma (EDTA anticoagulant recommended). Separate serum or plasma from blood within 3 hours of collection. Obtain a new specimen if the original is severely hemolyzed. Ideally, test immediately after specimen collection. If not possible, store the specimen at 2–8℃ for up to 72 hours or below -20℃ for long-term storage. Bring all materials to room temperature before use. Thaw frozen specimens completely and mix well. Avoid repeated freezing and thawing. Use only clear, non-hemolyzed specimens.

Test Procedure

Refer to the Immunofluorescence Analyzer Operation Manual for detailed instructions. Place the Test Device on a clean, level surface. Insert the ID Chip into the analyzer and click “Read ID Chip.” Ensure the Test Device lot number matches the ID Chip number. Pipette 50 μl of prepared sample into the cProgesterone Sample Buffer and mix gently to avoid vigorous agitation and foaming. Pipette 100 μl of the mixed sample into the sample well (S) of the Test Device. Avoid bubbles. Select the Test Mode: Standard Test : Click “Standard Test,” insert the Test Device into the analyzer immediately after adding the sample, select “Serum/Plasma,” and start the test. The meter will automatically read and display the result. Quick Test : Click “Quick Test,” start the timer after adding the sample mixture, incubate the Test Device at room temperature for 10 minutes , then insert the device into the analyzer, select “Serum/Plasma,” and start the test. The result will be displayed automatically. Results will be shown on the screen and can be printed either automatically or by clicking “Print.”

Quality Control

Each cProgesterone Rapid Quantitative Test includes an internal control for routine quality control. If the internal control result is invalid, the analyzer will display an error message prompting a retest.

Interpretation of Results

Detection Range : 1.0 ~ 50 ng/ml Conversion Factor : 1 ng/ml = 3.18 nmol/L Result (ng/ml) Result (nmol/L) Level Suggestion ≤ 1.0 ≤ 3.18 Negative Non-estrus phase

• 1.0 - 5.0
• 3.18 - 15.9 Low Preovulation phase
• 5.0 - 10.0
• 15.9 - 31.8 Medium+ Oviposition phase
• 10.0 - 20.0
• 31.8 - 63.6 Medium++ Optimal fertility phase

20.0 63.6 High+++ Ootid senescence phase Note: A result above Level 1 in an unvaccinated pet may indicate past infection. Each laboratory should establish its own reference range based on the specific population being evaluated.

Warnings and Limitations

For in vitro diagnostic use only . Inspect packaging and labels before use. Do not use if the pouch is damaged, improperly sealed, or if the vial is damaged or leaking. Do not use the test device beyond its expiration date. Use one pipette tip per specimen to prevent cross-contamination. Technical or procedural errors and unlisted substances in blood may affect test results and lead to inaccuracies.