Migi (Cat) Feline Coronavirus Antigen (FCoV-Ag) Rapid Quantitative Test Kit

Catalog Number: MG-CA-R029

Product Specifications

Attribute	Value
Place of Origin	China
Classification	Specific Reagents
CAT No.	MG-CA-R029
Package	Box
Appearance	Liquid
Specification	10T
Storage	Room Temperature
Shelf life	2 years
MOQ	10 boxes
Suitable for	Veterinary
Grade	Reagent Grade

Specification

10 Tests/Box Cat. No.: MG-CA-R029

Intended Use

The FCoV-Ag test is a fluorescence immunoassay designed for use with an Immunofluorescence Analyzer to quantitatively determine feline coronavirus antigen concentration in feline rectal swabs. It serves as an aid in the diagnosis and monitoring of feline coronavirus infection. For in vitro diagnostic use only. For professional use only.

Clinical Significance

Feline coronavirus (FCoV) is ubiquitous in cat populations and, in a subset of infected animals, can mutate to cause feline infectious peritonitis (FIP) — a frequently fatal systemic disease. Because clinical signs are often vague in the early stages, antigen detection provides a valuable tool for identifying active infection and guiding management in catteries and shelters.

Quantitative FCoV antigen measurement assists in distinguishing active shedding from past exposure and supports screening programmes aimed at reducing environmental viral load, a key strategy for FIP prevention.

Test Principle

This test uses a quantitative double antibody sandwich fluorescence immunoassay technique. The intensity of the fluorescent signal correlates with the amount of FCoV-Ag captured, with the concentration expressed in Tu/ml.

Materials

Material Provided Test Kit : Test Device Desiccant pouch 10 sealed pouches, each containing: ID Chip Instructions for Use 10 Cotton Swabs 10 Tubes of FCoV-Ag Sample Buffer 10 Pipette Tips Material Required But Not Provided Immunofluorescence Analyzer Timer Pipette Centrifuge

Storage and Stability

Store the test kit at 4～30℃ until the expiration date. Operate the test at 18 ~ 28℃ after opening the Test Device. Perform the test within 30 minutes of opening the pouch.

Specimen Collection and Preparation

The test is performed using a rectal swab. For sample collection: Insert the swab into the feline’s rectum and rotate it near the inner wall 3 ~ 5 times. The sample volume should cover about 1/2 ~ 2/3 of the cotton tip. Perform the test immediately after specimen collection. If testing cannot be done within 3 hours, store the specimen at 2～8℃ for up to 48 hours. For long-term storage, keep the specimen below -20℃. Before use, bring all materials to room temperature. Completely thaw and mix frozen specimens well prior to testing. Avoid repeated freezing and thawing. Use only clear, non-hemolyzed specimens.

Test Procedure

Refer to the Immunofluorescence Analyzer Operation Manual for detailed instructions on using the Test Device. Place the Test Device on a clean, level surface. Insert the ID Chip into the meter and click “Read ID chip.” Ensure that the Test Device lot number matches the ID Chip number. Insert the rectal swab with the sample into the FCoV-Ag Sample Buffer. Stir thoroughly and centrifuge for 1 minute (3000 ~ 5000 rpm). Pipette 100 μl of the mixed sample into the sample well (S) of the Test Device. Avoid forming bubbles. Choose one of the following test modes: Standard Test : Click “Standard Test,” insert the Test Device into the meter holder immediately after adding the sample, and click “Start Test.” Select the sample type “Rectal Swab.” The meter will automatically count down and display the test result. Quick Test : Click “Quick Test,” start the timer immediately after adding the sample mixture to the sample well, and leave the Test Device at room temperature (18 ~ 28℃) for 10 minutes. Then, insert the Test Device into the meter holder and click “Start Test.” Select the sample type “Rectal Swab.” The instrument will automatically scan the Test Device and display the result. Results are displayed on the main screen and can be printed automatically or manually by clicking “Print.”

Quality Control

Each FCoV-Ag Rapid Quantitative Test contains an internal control for routine quality control. This internal control is performed each time a patient sample is tested. If an invalid result occurs, the meter will display an error message, indicating that another test should be conducted.

Interpretation of Results

Reference range of FCoV-Ag in feline rectal swabs: Detection range:

• 5.0 ~ 320 Tu/ml Reference range: Result (Tu/ml) Interpretation Suggestion ≤ 10 Negative (-) 10 ~ 12 Suspected (±) 12 ~ 20 Weak Positive (+) 20 ~ 80 Medium Positive (++)

80 Strong Positive (+++) Each laboratory should establish a reference range representative of the population being evaluated.

Warnings and Limitations

This kit is for in vitro diagnostic use only. Inspect packaging and labels before use. Do not use if the pouch is broken, torn, not well sealed, or if the vial appears damaged or leaking. Do not use the test device beyond the expiration date. Use a new pipette tip for each specimen. Technical or procedural errors, as well as other substances in blood specimens not listed in the instructions, may interfere with the test and result in erroneous outcomes.